To calm the bees when you open the hive, spray 1 to 2 squirts of undiluted EM Kin Probien directly over the bees. You should also spray the alighting boards. At the same time, the acid environment created by undiluted EM Kin Probien gives the bees initial protection against the varroa mite. Can be sprayed directly onto the honeycombs and does not leave an odour.